What does dark field microscopy primarily highlight?

Dark field microscopy is a specialized technique that significantly enhances the visibility of structures that are difficult to detect with conventional bright field microscopy. In dark field microscopy, light is directed at an angle so that it does not enter the objective lens directly. Instead, it illuminates the specimen in such a way that only the light scattered by the specimen is captured, creating a contrast where light appears against a dark background.

This technique is particularly effective for visualizing transparent organisms and very small objects that do not emit or reflect light strongly. In this context, option B accurately describes the fundamental principle of dark field microscopy, which relies on producing an image by highlighting structures with scattered light against a predominantly dark background. This allows for detailed observation of cellular components, microorganisms, and other minute structures that might otherwise go unnoticed.